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What is Phosphoric Acid ?

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• Phosphoric acid (H3PO4) is attached to each sugar at the 3′ and 5′ C

positions to give rise to the sugar-phosphate backbone. Free

nucleotides in the cell have 3 phosphate residues, generally attached

to the 5′ C of the pentose. During the phosphodiester bond

formation, two phosphate groups are removed from one of the two

participating nucleotides.

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