The DNA molecule contains palindrome nucleotide sequences, identical when read in both directions as 5'-3' or 3'-5'. Restriction enzymes act at these sites.
Below are five palindromic DNA sequence examples:
i. 5' GGATCC 3' ---------3' CCTAGG 5' ---------
ii. 5' AAGCTT 3' ---------3' TTCGAA 5' ---------
iii. 5'ACGCGT 3' ---------3' TGCGGA 5'--------- 5' ACGCGT 3' -------- 3'-- ----- TGCGCA 5'
iv. 5' ACTAGT 3' --------- 3' TGATCA 5' --------- v. 5' AGGCCT 3' --------- 3' TCCGGA 5' --------