• Tissues from leaves, stems, roots and inflorescences can be directly cultured to
produce plant organs. In direct organogenesis, the tissue undergoes morphogenesis
without going through a callus or suspension cell culture stage. The term direct
adventitious organ formation is also used for direct organogenesis.
• For appropriate organogenesis in culture system, exogenous addition of growth
regulators—auxin and cytokinin is required. The concentration of the growth
promoting substance depends on the age and nature of the explant, besides the growth
conditions.