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What is Direct Organogenesis?

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• Tissues from leaves, stems, roots and inflorescences can be directly cultured to

produce plant organs. In direct organogenesis, the tissue undergoes morphogenesis

without going through a callus or suspension cell culture stage. The term direct

adventitious organ formation is also used for direct organogenesis.

• For appropriate organogenesis in culture system, exogenous addition of growth

regulators—auxin and cytokinin is required. The concentration of the growth

promoting substance depends on the age and nature of the explant, besides the growth

conditions.

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