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Technique of Micro propagation:

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• Micro propagation is a complicated process and mainly involves 3 stages

(I, II and III). Some authors add two more stages (stage 0 and IV) for more

comprehensive representation of micro- propagation.

• Stage 0:

• This is the initial step in micro- propagation, and involves the selection

and growth of stock plants for about 3 months under controlled

conditions.

• Stage I: In this stage, the initiation and establishment of culture in a

suitable medium is achieved. Selection of appropriate explants is

important. The most commonly used explants are organs, shoot tips and

axillary buds. The chosen explant is surface sterilized and washed before

use.• Stage II:

• It is in this stage, the major activity of micro propagation occurs in a

defined culture medium. Stage II mainly involves multiplication of shoots

or rapid embryo formation from the explant.

• Stage III: This stage involves the transfer of shoots to a medium for rapid

development into shoots. Sometimes, the shoots are directly planted in

soil to develop roots. In vitro rooting of shoots is preferred while

simultaneously handling a large number of species.• Stage IV:

• This stage involves the establishment of plantlets in soil. This is done

by transferring the plantlets of stage III from the laboratory to the

environment of greenhouse. For some plant species, stage III is

skipped, and un-rooted stage II shoots are planted in pots or in

suitable compost mixture.

• The different stages described above for micro propagation are

particularly useful for comparison between two or more plant

systems, besides better understanding.

• It may however, be noted that not all plant species need to be

propagated in vitro through all the five stages referred above.

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